Bruker ultraflextreme maldi toftof mass spectrometer. Sites were provided with a test panel containing 10 organisms. To complete your malditof ms workflow concept based on a microflex maldi system a few consumables are required, such as a bacterial test standard bts, a hcca matrix hcca acyano4hydoxycinnamic acid and a small amount of an organic solvent. The microflex lrf is the best in class benchtop malditof and has been designed for more demanding applications.
Performances and reliability of bruker microflex lt and. The microflex ltsh maldi tof mass spectrometer with n 2 laser at 60 hz repetition rate the microflex ltsh smart system with bruker s proprietary smartbeam solidstate laser technology at 200 hz repetition rate, the first maldi tof system for microbial identification using a lifetime laser. In microbiology, it is being used as a rapid, accurate and costeffective method for the identification of microorganism bacteria, fungi and viruses. Whether it is biomarker discovery, oligonucleotide, small molecule or polymer analysis, or even the innovative fast an. How to convert bruker fid file to xzml or mzxml for. How brukers user manuals help you to successfully apply our advanced software and use our hardware. For malditof ms analysis, each isolate was smeared directly from a colony onto a 96well target plate in duplicate, using a sterile wooden stick or 1. A typical experiment consists of growth of the organism e. We carried out a side by side comparative evaluation of the performance of bruker biotyper versus vitek ms for identification of a large and diverse collection of.
Applied biosystemsmds sciex 4800 maldi toftof analyzer. Bruker microflex series innovative mass spectrometry. I am a frequent user of biomap but i couldnt get the data to load so i tried the create target and analyze this software since it is compatible with similar flex instruments but it still will not recognize the file formats. Maldiims for spatial analysis of lipids and other small molecules.
Bruker daltonik gmbh introduction microflex user manual, version 1. All of our bruker software manuals and operators guides are designed to make your business in everyday life run smoothly. Literature room download our latest brochures, app notes and poster notes. Brukers microflex malditof system has more than met the labs expectations. Malditof bactec phoenix mgit manual lis integrate microbiology challenges monitor enhance workflow. From the overnight appropriate agar plates a portion or the whole colony was smeared onto the spots of vitek msds disposable target slides and then the spot was covered with 1. In clinical microbiology laboratories, routine microbial identification is mostly performed using culture based methodologies requiring 24 to 72 hours from culturing to identification. We compared the bd phoenix automated microbiology system to the bruker biotyper version 2. All isolates were run concurrently in duplicate on the bruker microflex lt and the vitek ms platforms. Im dealing with this issue with a malditof ultraflex iii bruker. After selecting a method, the voltage starts ramping up but when it reaches 2 kv it suddenly stops ramping and i get the. Many user friendly options available for making it an easy to use machine. Microflex ltsh from bruker daltonics selectscience.
Bruker daltonik gmbh table of changes version date changes remarks 1. July 29, 2008 topics covered sample requirements instrument overview technical general instrument use instructions calibration discussion tips and tricks software reference at end of powerpoint file will not be. Explore the latest products and resources related to your industry. Microlink manual, generic dtm manual, microflex rs485 converter manual, microflex product manuals, micropulser manual, ssv manual, pulse scope manual, field tools 24v supply manual, gch manual. Bruker corporation announces fda clearance to market the. A side by side comparison of bruker biotyper and vitek ms.
When the following prompt appears, just click on the logon button without changing anything. Malditof mass spectrometer microflex series bruker. Maldi biotyper is a modular system designed to be configured according to individual laboratory needs. The microflex lt is an affordable, benchtop malditof mass spectrometer for clinical proteomics, as well as for the routine analysis of peptides, proteins and other large molecules. Overview microflex series highest performance benchtop. Maldiims for spatial analysis of lipids and other small. The maldi biotyper based on the microflex tm ltsh maldi tof mass spectrometer with n 2 laser providing 60 hz repetition rate. Bruker offers laboratories the choice of the microbial maldi tof mass spectrometer that best fits their needs. Reproducibility reproducibility testing was performed at four sites. Matrixassisted laser desorption ionizationtime of flight mass spectrometry malditof ms has emerged as a rapid, highly accurate, and costeffective method for routine identification of a wide range of microorganisms.
Malditof mass spectrometry is a versatile analytical technique to detect and characterize mixtures of organic molecules. Below you find manuals related to several solutions. Bruker biflex iii malditof user training jonathan a. The microflex ltsh malditof mass spectrometer with n 2 laser at 60 hz repetition rate the microflex ltsh smart system with brukers proprietary smartbeam solidstate laser technology at 200 hz repetition rate, the first malditof system for microbial identification using a lifetime laser. The acquisition software will then finish loading and initializing the instrument. Validation and implementation of malditof ms in a public. The microflex ltsh is the affordable entrylevel member of the flex line of mass spectrometers that provide quick and reliable results from the smallest sample amounts. The benchtop design allows for high maldi performance while using only little laboratory space. Bruker ultraflextreme malditoftof mass spectrometer. The bruker ultraflextreme utx mass spectrometer provides 2 khz speed in tof mode and 1 khz speed in toftof mode. Comparison of the bruker biotyper and vitek ms matrix.
Bruker autoflex maldi tof shared equipment authority. Designed for applications in need of a short time to result, the microflex smart ls features a fast solidstate bruker smartbeam laser and high performance vacuum system. The maldi biotyper ca system includes the benchtop microflex malditof matrixassisted laser desorption ionization timeofflight mass spectrometer, software, ivd labeled reagents, a. It is also capable of tandem maldims for peptide sequencing and protein identification. Each of our systems have been tested and backed with our standard warranty.
If you have further questions, please feel free to contact the bruker. Malditof bruker microflex lrf malditof bruker microflex lrf basic information. The maldi can be operated in reflectron mode for highresolution mass analysis of peptide and lower molecular weight polymers, and in linear mode for analysis of large molecules e. Mass range up to 300,000 da up to 10,000 da in reflectron mode workhorse instrument for analysis of synthetic polymers. Maldi is ideal for polymers, peptides, intact proteins and a wide variety of compounds. Bruker microflex maldi tof mass spec available at the lab world group a seller of used lab equipment. The autoflex is equipped with a maldi matrix assisted laser desorption ionization source. The direct transfer procedure recommended by the manufacturer was used to identify strains with the microflex lt. Target plate loaded moves to position pulls vacuum. Direct method sample is applied to target plate toothpick pipette tip matrix is overlaid on sample. Together with the dxm microscan walkaway system, the bruker maldi biotyper can provide a rapid and accurate solution for id and ast testing. A portion of a colony in the exponential growth phase was smeared onto a 96well target plate, and after drying, it was covered using 1. The product is best suited for imaging purpose in metabolomics and proteomics. Sensitivity even for a minute amount of samples and impressive mass resolution over a wide mass range makes the new ultraflex ii the instrument of choice for highsuccess expression proteomics and advanced biomarker discovery studies.
With this technology, the lab has expanded identification capabilities to other organism groups and added direct presumptive identifications of organisms from positive blood cultures the same day the aerobic andor anaerobic blood culture bottles become positive. Bts and hcca are sent as dry components in small readytouse vials and are reconstituted with organic solvent upon need. The microflex ltsh is a true benchtop solution taking up less than 1 meter 40 of counter space to provide flexibility in meeting laboratory needs. Comparison of the vitek ms and bruker microflex lt maldi. The microflex ltsh is a compact linearmode benchtop maldi tof system designed as an easytouse, but high performance solution for basic applications. It is designed to help you quickly learn how to use the 4800 maldi toftof analyzer. Bruker nanoelute is designed with years of proteomics knowhow and nanouhplc expertise to deliver robust and reliable operation from the. For masses below 5000 da the instrument provides unit resolution when operated in the reflection mode. With just a few clicks youll get the documentation you need. Bruker daltonics microflex lt malditof ms speciation. Brukers patented smartbeam technology is already widely accepted as a superior maldi imaging laser technology. The bruker maldi biotyper system is an industryleading microbiology system used for rapid microbial identification. When operated in the linear mode the autoflex has an upper mass range of 100 kda.
Laser focus diameters down to 10um for high spatial resolution imaging without pixel overlap. Comparison of bruker biotyper matrixassisted laser. High mass accuracy 12,000 mass range up to 20,000 mz equipped with agilent 1100 hplc system for separation and characterization of mixtures combination of accurate mass measurement and isotopic pattern for molecular formula verification. The instrument is capable of performing analysis of synthetic polymers, although the primary. The ultraflex ii also incorporates a t3sequencing capability, a novel and unique. Werecommendusing sdhbmaldimatrixinsteadof2,5dhbformaldi tof msanalysisofverylargeproteinsand. One microliter of supernatant from each sample was deposited onto a spot on a polished steel msp 96target plate bruker daltonics, inc. Bruker maldi biotyper microbiology systems beckman coulter. The microflex offers high resolution reflectron mode for compounds less than 5,000 da and low resolution linear mode for compounds greater than 5,000 da. Technological innovations, prominent manufacturers and popular equipment all in one place. Spots were completely air dried, and, then, the slides were placed on an adapter and inserted to vitek ms instrument. We have great deals on used lcms and mass spectrometers from sciex, thermo scientific, agilent, bruker, shimadzu and more. Bruker microflex series innovation mass spectrometry systems by bruker corporation.